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Epigenetic immune cell counting in human blood samples for immunodiagnostics
Abstract Full text Reprint
PRECISION FOR MEDICINE’S EPIGENETIC IMMUNE CELL DIAGNOSTIC TOOL EPIONTIS ID™ SHOWS PROMISE IN DETECTING SEVERE IMMUNE DISEASES NOT CURRENTLY IDENTIFIED IN NEWBORN SCREENINGS
New Research Finds Immune Monitoring Technology Could Advance Newborn Screening as well as Treatment of HIV Patients in Low-Resource Countries
BETHESDA, MD – August 1, 2018 – A novel diagnostic approach using epigenetic immune monitoring to screen newborns for inherited diseases could expand the number of life-threatening immune deficiencies identified in newborns. This method could enable treatment to begin soon after birth, promising improved disease outcomes and survival. The research is published in the August issue of the journal, Science Translational Medicine.
“While further research is needed, these initial results are very encouraging as they provide early evidence that this epigenetic technology could eventually be a newborn screening method that would identify primary immune diseases that are currently very difficult to detect,” said Rosa Bacchetta, M.D., one of the study investigators and an Associate Professor, Department of Pediatrics, Stanford School of Medicine. “Typically, children are not diagnosed until they’re sick and are showing symptoms at which point there already is organ impairment and infections that make effective treatment much more difficult. It would be highly advantageous if we could identify these diseases at birth and begin treatment soon after birth, prior to the development of symptoms.”
Improving Care for HIV Patients
The advantages that this epigenetic technology has in quantifying immune cells from dried blood was also shown to have additional positive implications for the treatment of HIV infections. Current methods for counting immune cells, such as those done with flow cytometry, require fresh or well-preserved blood which is especially challenging for monitoring HIV patients in limited-resource areas where lab facilities may be far away from where a patient lives and is treated. The research found that immune cell counts could be effectively done using the epigenetic technology on a dried blood sample, which can then be communicated back to the patient’s health practitioner for proper medication adjustments.
“This technology is simple to use and inexpensive, making it especially promising for improving the care of HIV patients where monitoring blood cell counts is critical to proper treatment but extremely difficult to do in parts of the developing world where storing and transporting liquid blood is not feasible, said Sven Olek, PhD, a study investigator and managing director of Epiontis GmbH, a German subsidiary of Precision for Medicine that developed Epiontis ID, the epigenetic qPCR used in the research. “Since a drop of the patient’s blood can be placed on a piece of paper and mailed into a lab for analysis, this technology eliminates a patient’s need to travel, often long distances, to a facility to have blood drawn. This increases the probability that the patient’s immune cell counts will be more consistently monitored.”
Epigenetic quantitative real-time PCR (qPCR) assays were developed for analysis of human leukocyte subpopulations. Whole blood from 25 healthy donors, 97 HIV positive patients, as well as DBS from 250 healthy newborns and 24 newborns with primary immunodeficiencies were analyzed.
Concordance between flow cytometric and epigenetic data for neutrophils, B, NK, and CD3 positive T cells, CD8 positive T cells, CD4 positive T cells, and FOXP3 positive T regulatory cells was evaluated demonstrating substantial equivalence between epigenetic quantitative PCR analysis and flow cytometry. Epigenetic qPCR achieved both relative and absolute quantification.
Applied to DBS, epigenetic immune cell quantification was shown to identify newborns suffering from various primary immunodeficiencies.
Ongoing Research Efforts
To widen the clinical applicability of epigenetic immune monitoring in pediatric medicine, research is currently being conducted on cohorts of healthy children to provide more precise diagnostic thresholds for detecting immunologic problems, including autoimmune dysregulations.
About Precision for Medicine
Precision for Medicine supports life sciences companies in the use of biomarkers essential to targeting patients more precisely and effectively. Precision applies novel biomarker approaches to clinical research that take advantage of the latest advancements in science and technology, focusing predominantly on genomics, immune-response assays, global specimen logistics, biomarker analytics, companion diagnostics, and clinical trial execution. Precision’s immune monitoring solutions include its proprietary molecular diagnostics technology, Epiontis ID™. Precision for Medicine is part of Precision Medicine Group, with more than 1,300 employees in 25 locations in the US, Canada, and Europe. For more information, visit precisionformedicine.com.
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