Translational Research - Biomarkers - Assays
Immunohistochemistry in Precision Medicine: From Biomarker to Clinical Trial Assay
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CELL TYPE(S) | ASSAY EXAMPLES |
---|---|
Whole blood |
Inhibition of LPS stimulated TNFα and IL-6 release Stimulation (PHA) and inhibition (CsA) of cell proliferation |
PBMCs |
Inhibition of OKT3 (CD3) stimulated IL-2 and IFNγ release Inhibition of CD3:CD28 stimulated TNFα release |
Monocytes & Monocyte-derived Macrophages |
Inhibition of LPS stimulated TNFα release |
Lymphocytes |
Inhibition of OKT3 (CD3) stimulated IL-2 release |
Neutrophils |
Transmigration (+HUVEC) Superoxide release |
HUVECs |
Inhibition of thrombin stimulated IL-8 and MCP-1 release |
Hepatocytes |
Inhibition of TNFα stimulated IL-6 and IL-8 release CYP induction (mRNA) |
Stellates |
TGF-β1-induced changes in fibrogenic gene expression and collagen deposition |
Alveolar macrophages |
Inhibition of LPS stimulated TNFα release |
Keratinocytes |
Stimulation (IL-4) and inhibition (CsA) of cell proliferation |
Synovial fibroblasts (RA) |
Validated assay for screening novel anti-TNFα therapeutic antibodies (biosimilars) |
Details:
Assay development included:
Assay validation included:
Precision also performed a biosimilar screen comparing relative potency vs Humira.
Results
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Translational Research - Biomarkers - Assays
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