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Single-cell Sequencing

Single-cell sequencing reveals the full complexity of cellular diversity compared to bulk sequencing.
In-depth single-cell sequencing allows easy and efficient exploration of the cellular functions of individual cells. Use single-cell sequencing to explore the landscapes of malignant cells and immune cells, tumor heterogeneity, and the underlying mechanisms of tumor biological behaviors in your clinical trial. 

Customized single-cell sequencing solutions

Precision for Medicine provides an end-to-end package of single-cell sequencing solutions, making use of the latest technology on the market: the 10x Genomics Chromium system and Illumina NGS platform. We offer high-throughput and high-accuracy single-cell sequencing service, coupled with a fast turnaround time.
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Single-Cell Gene Expression

Profiling of cellular heterogeneity, novel targets, and biomarker in each cell from a heterogeneous cell population

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Single-Cell Immune Profiling

Analyze full-length, paired B-cell or T-cell receptors, antigen specificity, and gene expression, all from a single cell

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Biomarker Discovery for Specific Cell Subpopulations

Characterize cell populations without prior knowledge of cell subtypes or cell markers

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Multiomic Profiling – Cell Surface Protein

Simultaneously measure cell surface protein expression and correlate gene expression measurements with immunophenotyping at a single-cell level to better delineate immune cell subsets and functional states

 

 

 

 

 

 

 

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Novel Cell Type and Cell State Discovery

Identify or interrogate:

  • Rare subpopulations
  • Circulating tumor cells (CTCs)
  • Tumor or immune microenvironment
  • Tumor heterogeneity and molecular subtype
  • Mechanisms associated with tumorigenesis, progression, metastasis, evolution, relapse, and therapy resistance
  • Cancer stem cells (CSCs)
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Diverse Sample Compatibility

  • Whole blood
  • PBMCs
  • FFPE tissues
  • Frozen tissues
  • Fresh tissues

 

 

 

 

 

 

Single-Cell Sequencing Workflow

Sample QC

Sample preparation

Follow best practices for washing, counting and concentrating cells to minimize presence of cellular aggregates and retrieve high-quality single cell suspensions.

Sample QC

Library QC

Single Cell Library Preparation
  • Platform: 10x Genomics Chromium X
  • GEM generation and barcoding
  • Post GEM-RT cleanup
  • cDNA amplification
  • Library construction

Library QC

Data QC

Sequencing

Platform: Illumina NovaSeq 6000

Data QC

Bioinformatics Analysis

Software: 10x Genomics Cell Ranger

Frequently Asked Questions

What is single-cell sequencing?

Single-cell sequencing is a next-generation sequencing method involving single-cell isolation, transcriptome characterization, and sequencing library generation. Single-cell sequencing uncovers heterogeneity at a high resolution by revealing cellular gene expression, quantity, and diversity in a sample.

What advantages does single-cell sequencing have over traditional methods?

The main difference between single-cell sequencing and traditional methods of bulk sequencing is that single-cell sequencing allows for characterization of heterogeneous samples by profiling the whole transcriptome at a single-cell resolution. Single cell is often used in conjunction with traditional methods of phenotyping such as flow cytometry or multiplexed immunofluorescence to obtain a comprehensive understanding of samples.

Can I customize the assay to fit my specific research needs?

Yes! We understand that no two research projects are exactly alike. We work with you to understand your particular research needs, and our team provides cost-effective recommendations to accomplish your goals. Contact our team today for more information about custom assay development and specialized bioinformatics support.

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