Single-Cell
Sequencing
Single-cell sequencing reveals the full complexity of cellular diversity compared to bulk sequencing. It is primarily used to address challenges in the study of uncommon cell types, such as circulating tumor cells, cell-lineage associations, and heterogeneous samples. In-depth single-cell sequencing allows easy and efficient exploration of the cellular functions of individual cells. Use single-cell sequencing to explore the landscapes of malignant cells and immune cells, tumor heterogeneity, and the underlying mechanisms of tumor biological behaviors in your clinical trial.
Precision for Medicine provides an end-to-end package of single-cell sequencing solutions, making use of the latest technology on the market: the 10x Genomics Chromium system and Illumina NGS platform. We offer high-throughput and high-accuracy single-cell sequencing service, coupled with a fast turnaround time.
Customized solutions to meet your needs
Single-Cell Gene Expression
Profiling of cellular heterogeneity, novel targets, and biomarker in each cell from a heterogeneous cell population
Single-Cell Immune Profiling
Analyze full-length, paired B-cell or T-cell receptors, antigen specificity, and gene expression, all from a single cell
Multiomic Profiling –
Cell Surface Protein
Simultaneously measure cell surface protein expression and correlate gene expression measurements with immunophenotyping at a single-cell level to better delineate immune cell subsets and functional states
Novel Cell Type and
Cell State Discovery
Identify or interrogate:
- Rare subpopulations
- Circulating tumor cells (CTCs)
- Tumor or immune microenvironment
- Tumor heterogeneity and molecular subtype
- Mechanisms associated with tumorigenesis, progression, metastasis, evolution, relapse, and therapy resistance
- Cancer stem cells (CSCs)
Biomarker Discovery for
Specific Cell Subpopulations
Characterize cell populations without prior knowledge of cell subtypes or cell markers
Diverse Sample Compatibility
- Whole blood
- PBMCs
- FFPE tissues
- Frozen tissues
- Fresh tissues
Single-Cell Sequencing Workflow
Frequently Asked Questions
Single-cell sequencing is a next-generation sequencing method involving single-cell isolation, transcriptome characterization, and sequencing library generation. Single-cell sequencing uncovers heterogeneity at a high resolution by revealing cellular gene expression, quantity, and diversity in a sample.
The main difference between single-cell sequencing and traditional methods of bulk sequencing is that single-cell sequencing allows for characterization of heterogeneous samples by profiling the whole transcriptome at a single-cell resolution. Single cell is often used in conjunction with traditional methods of phenotyping such as flow cytometry or multiplexed immunofluorescence to obtain a comprehensive understanding of samples.
Yes! We understand that no two research projects are exactly alike. We work with you to understand your particular research needs, and our team provides cost-effective recommendations to accomplish your goals. Contact our team today for more information about custom assay development and specialized bioinformatics support.
Related Resources
Methods for a Comprehensive Characterization of the Tumor Microenvironment
Precision for Medicine, June 2023.
The Significance of Genomics Analysis in Gene Therapy Development
Jie Yang, PhD, 2022.
Precision for Medicine Genomics Platforms and Solutions
Precision for Medicine, 2022.
Guiding Precision Medicine With Liquid Biopsy: Circulating DNA vs Circulating Tumor Cells
Jesus Garcia, PhD, 2022.
Additional Specialty Lab Services
Tissue & Liquid Biopsy
Obtain a detailed molecular view into patient biology with a range of tissue and liquid biopsy technologies, including our proprietary ApoStream® platform, which can isolate and enrich for circulating tumor cells for downstream analysis.
Bioanalytical Testing
Get bioanalytical support for your biologic, cell therapy, gene therapy, or companion diagnostics program from the globally recognized leaders in immunogenicity testing.
Genomics
Find and monitor gene expression signatures, copy number variations, chromosome rearrangements, and other genomic events occurring in patient samples using our NGS, NanoString, qPCR, ddPCR, and FISH/ISH services.
Precision’s Genomics Services are part of comprehensive end-to-end contract research and clinical development services
Translational
Central Labs
