Spectral
Flow
Cytometry
At Precision for Medicine, our flow cytometry specialists can help develop complex multicolor panels using conventional or spectral flow cytometry. Through the characterization of the immune response, flow cytometry as a tool for immune surveillance can facilitate the identification of biomarkers and exploration of immune correlates in immunotherapy trials.
Flow cytometry has enabled clinical trial development with cell identification and characterization, allowing for the simultaneous interrogation of multiple targets on various cell subsets from limited samples. To ensure the integrity of flow cytometry test specimens generated by global clinical trials, we have a worldwide network for PBMC and sample processing, logistics, and biostorage, and multiple specialty lab facilities in the US and in Europe.
Spectral flow cytometry applications
High-throughput, multiplexed immunophenotyping
Detect antigens and therapeutic targets of interest with up to 64 parameters and comfortably interrogate up to 40 fluorophores simultaneously using high-dimensional spectral flow cytometry.
We provide a full range of flow cytometry assays, including:
- Rare cell detection and quantification based on phenotype, apoptosis, or phosphorylation status
- Receptor occupancy (RO) assays and ligand measurement
- CAR NK-/T-cell therapy expression, characterization, persistence, and immunogenicity
- Phospho-flow and intracellular (ICC) flow
- Antigen-specific flow—detection and characterization, peptides, dextramers, tetramers
- Modulation of activation and exhaustion markers
- Immunophenotyping and absolute counting of cell subsets
- Cytokine measurement using cytometric bead assays
- Apoptosis/Cell cycle analysis/Histone methylation
- Custom flow cytometry biomarker assays
- GCP/GLP regulated assays
Customized solutions for your objectives
Collaborate with our experienced Precision for Medicine flow cytometry teams to custom design flow cytometry assays for your research and clinical studies.
What is spectral flow cytometry?
Spectral flow cytometry captures the full emission spectrum of fluorescent molecules using arrays of highly sensitive light detectors and has enabled characterization of high-parameter flow cytometry in a single sample. By measuring the full spectrum of light emissions from each cell, spectral flow cytometry can differentiate a vast combination of fluorophores, increasing flexibility in application design and enabling the development of complex multicolor panels for comprehensive immunophenotypic analysis by flow cytometry.
Instrumentation
Cytek® Aurora 5-laser
The Cytek Aurora leverages full-spectrum technology to provide high-resolution data at the single-cell level to resolve challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity. At present, our Cytek instrument’s capabilities are
- 64 parameters
- 5 lasers (355, 405, 488, 561, 640nm)
Flow Cytometry Resources
Resources
Webinar: Flow Cytometry for Clinical Applications: Recommendations on Multi-site Assay Transfer and Method Validation
Deborah Phippard, PhD, and Laura Lozza, PhD. July 2022.
Poster: An Assay to Monitor the Engagement and Modulation of CD6 on T cells as a Clinical Biomarker of Treatment With Itolizumab
Angelina Bisconte, et al. AAI Immunology, 2021.
Moving from Exploratory to Clinical Flow Cytometry Assays: 3 Critical Considerations
Deborah Phippard, PhD. May 2023.
Choosing the Optimal Sample Type for Flow Cytometry: Key Considerations and Case Studies
Angelina Bisconte and Deborah Phippard, PhD. June 2023.
Values of Precision Flow Cytometry
Established experience running complex flow cytometry in cell therapy, immuno-oncology, hematology and rare disease therapies, validating up to CLIA
Sample testing by senior staff with >5 years of experience who understand the disease biology and gating strategy
Real-time quality control during the run to ensure sample integrity
Additional Specialty Lab Services
Tissue & Liquid Biopsy
Obtain a detailed molecular view into patient biology with a range of tissue and liquid biopsy technologies, including our proprietary ApoStream platform, which can isolate and enrich for circulating tumor cells for downstream analysis.
Bioanalytical Testing
Get bioanalytical support for your biologic, cell therapy, gene therapy, or companion diagnostics program from the globally recognized leaders in immunogenicity testing.
Genomics
Find and monitor gene expression signatures, copy number variations, chromosome rearrangements, and other genomic events occurring in patient samples using our NGS, NanoString, qPCR, ddPCR, and FISH/ISH services.
Precision’s Central Lab Services are part of comprehensive end-to-end contract research and clinical development services
Translational
Central Labs
