ELISpot and FluoroSpot: Antigen-Specific Response Assays

ELISpot and FluoroSpot are highly sensitive functional assays that can be used throughout preclinical and clinical development, including phase 3 clinical trials. These assays can be extremely useful for measuring antigen-specific T cells, which typically occur at low frequency in vivo. Both assays measure the cell-mediated immune response with accurate ex vivo frequency measurement of antigen-responsive cells down to the 1-in-1,000,000 cell range.

Precision offers both ELISpot and FluoroSpot assays to support global preclinical and clinical projects, providing pre-validation and validation services using standard and custom assays.

ELISpot and FluoroSpot: Antigen-Specific Response Assays

ELISpot and FluoroSpot are highly sensitive functional assays that can be used throughout preclinical and clinical development, including phase 3 clinical trials. These assays can be extremely useful for measuring antigen-specific T cells, which typically occur at low frequency in vivo. Both assays measure the cell-mediated immune response with accurate ex vivo frequency measurement of antigen-responsive cells down to the 1-in-1,000,000 cell range.

Precision offers both ELISpot and FluoroSpot assays to support global preclinical and clinical projects, providing pre-validation and validation services using standard and custom assays.

Evaluating cell-mediated immune responses

We support global preclinical research with FluoroSpot and ELISpot testing—measuring cytokine-secreting cells at the single-cell level
ELISpot assays

ELISpot assays provide robust measurement of cytokine-secreting cells at the single cell level. Like an ELISA, antibodies for the target cytokine are coated onto a 96-well plate. The cells being assessed are cultured in the same wells, stimulated to release cytokine, and then removed. Bound cytokines are visualized as with an ELISA, using chemiluminescence, with each spot corresponding to a single secreting cell.

FluoroSpot assays

FluoroSpot assays are similar to ELISpot assays but rely on detection using fluorescently labeled detection antibodies.

Fluorescent detection enables simultaneous measurement of cells secreting different cytokines through multiplexing.

Example ELISpot assay panels

Integrating biomarker and clinical trial data

To help researchers understand and interpret biomarker assay data in the context of other assays as well as the clinical trial data, Precision provides the option of managing and analyzing data using our proprietary QuartzBio Translational Informatics platform.

Learn more about the sample management, informatics, and computational capabilities of QuartzBio.

Global reach, multi-site support

Our FluoroSpot and ELISpot testing services support pre-clinical and clinical research, including multi-site studies, conducted anywhere in the world.

Working with Precision

Precision’s specialty lab scientists take a collaborative and consultative approach to projects and can provide recommendations on biomarker assay strategies and implementation. Services can be provided individually or as part of a comprehensive therapeutic development package including biomarker assays and clinical trials.

Related services

Cytokine Profiling

For an in-depth assessment of inflammatory responses, our cytokine profiling assays are designed to measure cytokines and chemokines released from chosen target cells. We use Luminex, MesoScale Discovery, and Quanterix SIMOA technology for sensitive detection in complex matrices, as well as ELISAs for single analytes.

Immune Monitoring by Flow Cytometry

Flow cytometry provides high-dimensional insight into immune cells and target engagement. We have both research-grade instruments for up to 20 parameters analysis, and CLIA instruments, all supported by a global sample processing and logistics network to ensure sample integrity.

Immune Monitoring by Epiontis ID

Precision’s proprietary Epiontis ID technology delivers robust, repeatable, yet cost-effective immune cell phenotyping without the need for viable cells. With over 100,000 clinical samples analyzed, Epiontis ID is a globally accepted immune monitoring technology.

Discuss your project requirements

Precision ELISpot and FluoroSpot assays can support your global preclinical and clinical projects, talk to our experts about your study needs.

Discuss your project requirements

Precision ELISpot and FluoroSpot assays can support your global preclinical and clinical projects, talk to our experts about your study needs.

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