| Function |
Measure unbound or unoccupied receptors on a cell using an unlabeled antibody and competing therapeutic antibody or drug-conjugated antibody |
Measure therapeutic antibodies bound to the receptor with an anti-therapeutic antibody detection reagent |
Measure binding of a therapeutic antibody to its receptor site and couple with binding of an alternative antibody that binds to a different site on the same receptor |
Measure effect of the therapeutic antibody binding on the function of the target receptor |
Measure biological effects of the therapeutic antibody binding to the target receptor |
| Clinical Significance |
Typically used for antagonistic therapeutic drugs that block ligand binding to the receptor |
Often applied when monoclonal antibodies to the receptor are not readily available or conjugation of the therapeutic itself may compromise binding to its receptor target |
Useful where receptor level or cell numbers can change over time |
Receptor shedding or inhibition/activation of receptor internalization over time or with increasing drug dose |
Cell proliferation or cytokine production, which may have implications in drug safety/efficacy |
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