Custom Immune Monitoring Assay Development

Precision offers exceptional scientific depth to support the development of de novo assays, with a particular specialization in cell-based assays and epigenetic immune cell phenotyping using our proprietary and patented Epiontis ID platform. We work with your scientific team to understand your needs-e.g. assay development, pre-clinical studies, clinical trial support-and then tailor an assay to achieve your objectives.

Custom Immune Monitoring Assay Development

Precision offers exceptional scientific depth to support the development of de novo assays, with a particular specialization in cell-based assays and epigenetic immune cell phenotyping using our proprietary and patented Epiontis ID platform. We work with your scientific team to understand your needs-e.g. assay development, pre-clinical studies, clinical trial support-and then tailor an assay to achieve your objectives.

Representative assays include:

  • ELISpots and Fluorospots
  • T cell activation and proliferation
  • Recall assays (e.g. response to Tetanus toxoid)
  • T cell polarization of naive T cells (Th1, Th2, Th17, Treg)
  • Mixed Lymphocyte reaction (MLR)
  • Antibody-dependent cell-mediated (ADCC) cytotoxicity
  • NK cell killing
  • Dendritic cell generation, isolation, and characterization
  • Macrophage differentiation
  • Neutrophil migration
  • Viable cell sorting – single cell or populations to support down-stream assays
  • Receptor Occupancy
  • Tetramer flow cytometry
  • Molecular phenotyping with Epiontis ID

Custom assay design: Novel approaches solve complex problems

Because the purpose of clinical development is to bring new therapies to market, studies often need to find answers for previously unasked questions, questions that don’t fit neatly into one specific method or approach. These are the projects Precision thrives on, devising clever and practical solutions for new challenges.

One example is illustrated in the diagram below where we addressed a client’s need to generate multiple types of biomarker analyses from a single skin punch biopsy. We were able to demonstrate that we could store the biopsy in RNAlater, section the tissue into thirds, and ship to multiple facilities and still obtain accurate and reliable biomarker data.

In the above example, each skin-punch sample was analysed using immunofluorescence and H&E staining, RNA-seq to understand gene expression (Nanostring was also an option), and Epiontis ID for immune cell profiling. Through this custom assay we were able to determine the spatial

distribution and numbers of specific immune cells, and integrate this information with their mRNA signatures, efficiently uncovering complex molecular insights into patient biology that would have been otherwise difficult to obtain.

Global reach, multi-site support

Our custom assay services support pre-clinical and clinical research, including multi-site studies, conducted anywhere in the world.

Working with Precision

Precision’s specialty lab scientists take a collaborative and consultative approach to projects and can provide recommendations on biomarker assay strategies and implementation. Services can be provided individually or as part of a comprehensive therapeutic development package including biomarker assays and clinical trials.

Related services

Immune Monitoring by Epiontis ID

Precision’s proprietary Epiontis ID technology delivers robust, repeatable, yet cost-effective immune cell phenotyping without the need for viable cells. With over 100,000 clinical samples analyzed, Epiontis ID is a globally-accepted immune monitoring technology.

Gene Expression Profiling of Biopsies

Understand target expression, adaptive and innate immune responses at the gene expression level. Technologies we use for gene expression profiling include NanoString, Illumina RNA-seq, ddPCR, and qPCR.

Cytokine Profiling

For an in-depth assessment of inflammatory responses, our cytokine profiling assays are designed to measure cytokines and chemokines released from chosen target cells. We use Luminex, MesoScale Discovery, and Quanterix SIMOA technology for sensitive detection in complex matrices, as well as ELISAs for single analytes.

Discuss your project requirements

Whether you know exactly the type of immune monitoring approach you need or would like expert support with project design, we can deliver

Discuss your project requirements

Whether you know exactly the type of immune monitoring approach you need or would like expert support with project design, we can deliver

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