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Custom Immune Monitoring Assay Development

Precision excels in developing immune monitoring assays to solve novel challenges, or assays tailored to your specific objectives

Precision offers exceptional scientific depth to support the development of de novo assays, with a particular specialization in cell-based assays and epigenetic immune cell phenotyping using our proprietary and patented Epiontis ID® platform.

  • Representative assays include:

  • fluorescence

    • ELISpots and FluoroSpots
    • T cell activation and proliferation
    • Recall assays (eg, response to Tetanus toxoid)
    • T cell polarization of naive T cells (Th1, Th2, Th17, Treg)
    • Mixed lymphocyte reaction (MLR)
    • Antibody-dependent cell-mediated (ADCC) cytotoxicity
    • NK cell killing
    • Dendritic cell generation, isolation, and characterization
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    • Macrophage differentiation
    • Neutrophil migration
    • Viable cell sorting – single cell or populations to support downstream assays
    • Receptor occupancy
    • Tetramer flow cytometry
    • Molecular phenotyping with Epiontis ID®

Custom assay design: novel approaches solve complex problems

Because the purpose of clinical development is to bring new therapies to market, studies often need to find answers for previously unasked questions, questions that don’t fit neatly into one specific method or approach. These are the projects Precision thrives on, devising clever and practical solutions for new challenges.

One example is illustrated in the diagram below where we addressed a client’s need to generate multiple types of biomarker analyses from a single skin punch biopsy. We were able to demonstrate that we could store the biopsy in RNAlater, section the tissue into thirds, and ship to multiple facilities and still obtain accurate and reliable biomarker data.

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In the above example, each skin-punch sample was analysed using immunofluorescence and H&E staining, RNA-seq to understand gene expression (NanoString was also an option), and Epiontis ID® for immune cell profiling. Through this custom assay we were able to determine the spatial

distribution and numbers of specific immune cells, and integrate this information with their mRNA signatures, efficiently uncovering complex molecular insights into patient biology that would have been otherwise difficult to obtain.

Learn more about our Immune Monitoring Solutions

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Immune Monitoring Solutions

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Immune Monitoring Solutions
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Global reach, multi-site support

Our custom assay services support preclinical and clinical research, including multi-site studies, conducted anywhere in the world.

Working with Precision

Precision’s specialty lab scientists take a collaborative and consultative approach to projects and can provide recommendations on biomarker assay strategies and implementation. Services can be provided individually or as part of a comprehensive therapeutic development package including biomarker assays and clinical trials.

Additional Specialty Lab Services

  • Epiontis ID® Epigenetic Cell Profiling

    Precision’s proprietary immune monitoring technology which delivers robust, repeatable, and cost-effective immune cell phenotyping on any sample type
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  • Cytokine Profiling & Proteomics

    Customized assays to detect cytokines or proteins of interest utilizing ELISA, Luminex, MSD, SIMOA, and Western Blotting

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  • Gene Expression Profiling of Biopsies

    Understand target expression, adaptive and innate immune responses at the gene expression level. Technologies we use for gene expression profiling include NanoString, Illumina RNA-seq, ddPCR, and qPCR.
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Explore the Precision blog

Read our blog to gain more insights and discover work that Precision has supported.