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Flow Cytometry Analysis and Testing Services

Analyze cellular subpopulations of interest to shed light on biological events in your clinical studies

Our scientists develop complex multicolor panels, supporting sponsors based on context of use from exploratory, secondary, primary, and CLIA flow assays.

We routinely run complex assays, including receptor occupancy, CAR and cell therapy expression/function, antigen-specific flow, and phospho-flow. One core focus and area of expertise within our flow cytometry services is immunophenotyping. This is a crucial part of our comprehensive immune monitoring solutions, which are essential for multi-site clinical studies. These studies demand rigorous immunophenotypic analysis, particularly in trials for novel immunotherapies or cell therapies.

Flow Results Combo

Measure endpoints for a full range of applications

All assays are customizable and may be developed according to ISO/GCP/GLP/CAP/CLIA regulations.

  • Rare cell detection and quantification based on phenotype, apoptosis, or phosphorylation status

  • Immunophenotyping and absolute counting of cell subsets 

  • Modulation of activation and exhaustion markers

  • Apoptosis and cell cycle analysis via histone methylation

  • Cytokine production

For CAR NK-cell/T-cell therapy:

  • Expression of lineage-specific markers and characterization 
  • CAR T/NK persistence and immunogenicity

Approaches to leverage flow cytometry

  • Receptor Occupancy (RO)
  • Real-Time Flow
  • Prevalidated & Custom Panels
  • Flow Cytometry in Cell Therapy
  • Receptor Occupancy (RO)

    Understand how biotherapeutics engage their targets

    • Simultaneous detection of RO and other surface markers using multiparametric flow cytometry

    • Reagent specificity and sensitivity determined by extensive characterization during assay development

    • Optimal matrix selection: eg, whole blood or fixed whole blood

     

    Explore our receptor occupancy assay capabilities
    Occupancy Receptor Flow
  • Real-Time Flow

    Support clinical studies with whole blood and tissue flow analysis of clinical samples using custom or prevalidated panels

    • Sample tracking and accession with same-day/next-day flow sample processing

    • Global flow cytometry centers of excellence to support exploratory to GxP/CLIA sample processing

    • Rapid and accurate flow analysis by our flow experts

    • Assays are validated for intended use suitable for proof of concept, exploratory, secondary, or primary clinical trial endpoints.
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  • Prevalidated & Custom Panels

    Expedite clinical development with “off-the-shelf” panels
    • Validated fit-for-purpose flow assays using industry standards

    • Add additional markers to any panel to address key PD markers for your clinical study

    • Generate robust and reproducible data for clinical trials, and generate data for patient selection

    • Complex assays measuring antigen and cellular specific responses using tetramers, target specific peptides, and CAR-T/CAR-NK flow

     

    View our prevalidated flow cytometry panels
    Flow-Cytometry
  • Flow Cytometry in Cell Therapy

    Monitor the persistence and differentiation of cell therapy products

    • Evaluate CAR expression on therapeutic cells

    • Measure CAR T or CAR NK cell persistence and therapeutic effect

    • Assess cell functionality and potency of immune system response to cell therapy/CAR

     

    Explore our flow cytometry capabilities for cell therapy
    cell-therapy
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Leverage our expertise across flow cytometry assays

Precision routinely runs complex and custom assays for clinical trials under rigorous quality control procedures.

  • Intracellular cytokine secretion (ICS)

  • Receptor occupancy (RO) assays and ligand measurement

  • Phospho-flow for phosphorylation state

  • Immune functional assays: antibody-dependent cell-mediated cytotoxicity (ADCC), T-cell activation

  • Antigen-specific responses: detection and characterization of peptides, dextramers, tetramers

  • Cytokine measurement using cytometric bead assays

  • Histone methylation measurement

  • Spectral flow cytometry

Target the clinical biomarkers important in your trials

Our panels are carefully designed and curated, incorporating a trial-specific approach that combines expert input with years of flow cytometry experience. Customization is available.

  • Evaluate investigational agents in preclinical and clinical studies on a multi-site and worldwide basis
  • Perform biomarker discovery by identifying patient populations most likely to respond to therapy and monitoring pharmacodynamic changes on target cells in response to treatment
  • Customized sample collection kits and logistical services ensure optimal sample integrity

Precision range of flow cytometry instruments and capabilities can accommodate any project

BD FACSCanto IIBD FACSCanto™ IIBD Biosciences FACSAriaTM FusionBD FACSAria™ FusionLSRFortessaBD LSRFortessa™BD-Biosciences-FACSymphonyTM-A5BD FACSymphony™ A5Cytek Aurora (resized)Cytek Aurora
Parameter 10 18 20 31 64
Colors Up to 8 Up to 16 Up to 18 Up to 30 Up to 40
Lasers Up to 3 Up to 5 Up to 5 Up to 9 Up to 5
CLIA Assays Validated CLIA assays

Start with the appropriate sample type for your flow cytometry project

Consult with Precision to address the unique needs and challenges of your clinical trial, starting with sample choice, to ensure robust, reproducible flow cytometry data.

Flow Cytometry ApplicationsUntreated BloodPBMCFixed Blood

Immunophenotyping/Activation/Anergy

Vaccines/Antigen-Specific Tetramer

 

Peptide Flow/Intracellular Cytokine Secretion (ICS)

 

Cell Therapy Flow (CAR T, CAR NK)

PK Flow

 

Receptor Occupancy

 

Single Cell Sorting

Intracellular/Intranuclear Staining

Phospho-flow

 
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Ensure sample quality with global sample processing and specimen logistics

We use standardized processes that follow each sample from collection to analysis and our biorepositories can store fluid and solid biological specimens at controlled ambient temperatures, +4°C, -20°C, -80°C, and in liquid nitrogen.

  • Real-time inventory tracking via e-Portal dashboard

  • Global PBMC processing same day or next day 

  • Regular competency assessment and monitoring to ensure site-to-site and lab-to-lab data comparability

  • A biorepository with redundant back-up systems, dedicated back-up units and other safety and security features

Proven Results

Explore how researchers use Precision flow cytometry to power impactful science.

Explore more resources
  • Poster

    Validation of a Whole Blood and Proteomic Stabilized Blood Assay to Monitor the Engagement and Modulation of CD6 on T cells by Itolizumab as a Clinical Pharmacodynamic

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    Validation of a Whole Blood and Proteomic Stabilized Blood Assay to Monitor the Engagement and Modulation of CD6 on T cells by Itolizumab as a Clinical Pharmacodynamic
  • Poster

    An Assay to Monitor the Engagement and Modulation of CD6 on T cells as a Clinical Biomarker of Treatment with Itolizumab

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    An Assay to Monitor the Engagement and Modulation of CD6 on T cells as a Clinical Biomarker of Treatment with Itolizumab
  • Poster

    Evaluation of total PD-1 expression using multi-color flow cytometry in Metastatic Non-Small Cell Lung Cancer patients treated with Multi-Neoantigen Vector (ADXS-503) in

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    Evaluation of total PD-1 expression using multi-color flow cytometry in Metastatic Non-Small Cell Lung Cancer patients treated with Multi-Neoantigen Vector (ADXS-503) in
  • Poster

    Development and technical validation of tetramer staining for use as a biomarker for assessing gluten-specific T cells in clinical studies

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    Development and technical validation of tetramer staining for use as a biomarker for assessing gluten-specific T cells in clinical studies
  • Poster

    Validation of BTK & Aiolos Immunophenotyping Assay to Monitor B, NK & T cell Responses as a Clinical Biomarker in B-cell Malignancies

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    Validation of BTK & Aiolos Immunophenotyping Assay to Monitor B, NK & T cell Responses as a Clinical Biomarker in B-cell Malignancies

Values of Precision flow cytometry

Reproducible and valid flow cytometry data stems from experienced scientists and standardized protocols across multi-site programs. Leverage our deep network of expertise for your trials.
  • EstablishExperience

    Established experience

    Established experience running complex flow cytometry in gene therapy, cell therapy, immuno-oncology therapies, validating up to CLIA
  • SeniorStaf

    Senior staff

    Sample testing by experienced staff who understand the disease biology and gating strategy
  • QualityControl

    Quality control

    Real-time Quality Control during the run to ensure sample integrity
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Conduct trials globally with multi-site support for flow cytometry assays and analysis

We provide flow cytometry services that support preclinical and clinical research, including multi-site studies, conducted anywhere in the world. Samples are processed same day or next day and utilize proven SOPs to ensure sample integrity.

Collaborate with us for a personalized plan

Precision’s flow cytometry service scientists take a collaborative and consultative approach to projects and can provide recommendations on assay strategies and implementation, if needed. Services can be provided individually or as part of a comprehensive therapeutic development package including biomarker assays and clinical trials.