Flow Cytometry Services

Flow cytometry can provide incredibly rich data sets, shedding light on biological events at single-cell resolution. At Precision, we routinely run complex assays, and also can support sponsors with flow cytometry studies conducted on CLIA-certified instruments. One of the largest focuses of our flow cytometry services is for immune monitoring in support of global clinical trials;

To ensure the integrity of flow cytometry specimens generated by global clinical trials, we have a worldwide network for PBMC and sample processing, logistics, and biostorage, and multiple speciality labs facilities in the US and the EU.

Flow Cytometry Services

Flow cytometry can provide incredibly rich data sets, shedding light on biological events at single-cell resolution. At Precision, we routinely run complex assays, and also can support sponsors with flow cytometry studies conducted on CLIA-certified instruments. One of the largest focuses of our flow cytometry services is for immune monitoring in support of global clinical trials;

To ensure the integrity of flow cytometry specimens generated by global clinical trials, we have a worldwide network for PBMC and sample processing, logistics, and biostorage, and multiple speciality labs facilities in the US and the EU.

Flow cytometry applications

We provide a full range of flow cytometry assays including:

  • Cell profiling and subsetting
    • Lymphocytes (TH, TC, TM, Treg and B cells)
    • Stem cells (LSC and HSC)
    • Reticulocytes
    • Myeloid-derived suppressor cells (MDSC)
    • Macrophage and monocyte sub-populations
  • Rare cell detection and quantification based on phenotype, apoptosis or phosphorylation status
  • Receptor occupancy (RO) measurement
  • Intracellular staining (ICS)
  • Tetramer flow cytometry for antigen-specific flow cytometry
  • Cytokine measurement using cytometric bead assays
  • CAR-T cell characterization for cell therapy development
  • Cell sorting (FACS)
  • ISO/GCP/GLP/CAP/CLIA regulated assays

CLIA assays (BD FACSCanto™ Instruments)

Benefits of Precision’s flow cytometry services
  • Evaluate investigational agents in pre-clinical and clinical studies on a multi-site and worldwide basis.
  • Perform biomarker discovery by identifying patient populations most likely to respond to therapy and monitoring pharmacodynamic changes on target cells in response to treatment.
  • Customized sample collection kits and logistical services ensure optimal sample integrity
Global sample processing and specimen logistics to ensure sample quality

We use standardized processes that follow each sample from collection to analysis and a 100,000 sq. ft (9,300 sq. m.) primary biorepository that can store fluid and solid biological specimens at controlled ambient temperatures, +4°C, -20°C, -80°C, and in liquid nitrogen.

  • Real-time inventory tracking QuartzBio dashboard system
  • Global PBMC processing in 24-hours or less
  • Regular competency assessment and monitoring to ensure site-to-site and lab-to-lab data comparability
  • A biorepository with redundant back-up systems, dedicated back-up units and other safety and security features
Instruments

All of our flow cytometry studies are conducted on BD Biosciences systems:

  • BD LSRFortessa™
    • 20 parameters
    • 18 colors
  • BD FACSCanto™
    • 12 parameters
    • 10 colors
    • Supports CLIA assays
  • BD FACSCanto™
    • 10 parameters
    • 8 colors
    • Supports CLIA assays
  • BD FACSAria™ Fusion
    • 20 parameters
    • 16-18 colors
    • 4-way sorting
    • Tubes, 96-well plates, 384-well plates

Validation of a gliadin tetramer flow cytometric assay, a biomarker to assess gluten specific T cells in celiac disease

Precision excels at complex flow cytometry applications including tetramer flow cytomery. In this study, conducted with Takeda, we were able to validate an assay to detect rare tetramer cells in blood as a way to evaluate therapeutic response.

Global reach, multi-site support

We provide flow cytometry services that support pre-clinical and clinical research, including multi-site studies, conducted anywhere in the world. Samples are processed within 24 to 30 hours of draw and utilize standardized SOPs to ensure sample integrity.

Learn more about sample processing >

Working with Precision

Precision’s flow cytometry service scientists take a collaborative and consultative approach to projects and can provide recommendations on assay strategies and implementation, if needed. Services can be provided individually or as part of a comprehensive therapeutic development package including biomarker assays and clinical trials.

Integrating biomarker and clinical trial data

 

To help researchers understand and interpret biomarker assay data in the context of other assays as well as the clinical trial data, Precision can deliver flow cytometry and other biomarker data using our proprietary QuartzBio Learn More platform. QuartzBio features specific flow cytometry capabilities including interactive gating and visualization of high-dimensional data.

Learn more about the computational biology, informatics, and sample inventory management capabilities of QuartzBio

Additional specialty lab services

Tissue & Liquid Biopsy

Obtain a detailed molecular view into patient biology with a range of tissue and liquid biopsy technologies, including our proprietary ApoStream platform which can isolate and enrich for circulating tumor cells for downstream analysis.

Bioanalytical Testing

Get bioanalytical support for your biologic, cell therapy, gene therapy, or companion diagnostics program from the globally-recognized leaders in immunogenicity testing.

Genomics

Find and monitor gene expression signatures, copy number variations, chromosome rearrangements, and other genomic events occurring in patient samples using our NGS, NanoString, qPCR, ddPCR, and FISH/ISH services.

Discover how our flow cytometry expertise can advance your pre-clinical or clinical development program

Discover how our flow cytometry expertise can advance your pre-clinical or clinical development program

Explore other areas of expertise