Digital PCR (ddPCR) Services

Droplet Digital™ PCR is ideal for applications where high sensitivity and absolute quantification are desired. Digital PCR can also detect minute gene expression fold changes, and find rare genetic events in mixed cell populations.

Other benefits of Droplet Digital™ PCR include the ability to perform single-cell analysis, and the flexibility to analyze low abundance or challenging samples-such as cell-free DNA or environmental DNA.

Digital PCR (ddPCR) Services

Droplet Digital™ PCR is ideal for applications where high sensitivity and absolute quantification are desired. Digital PCR can also detect minute gene expression fold changes, and find rare genetic events in mixed cell populations.

Other benefits of Droplet Digital™ PCR include the ability to perform single-cell analysis, and the flexibility to analyze low abundance or challenging samples-such as cell-free DNA or environmental DNA.

Precision’s ddPCR capabilities and applications

Applications

Precision uses the Bio-Rad QX200™ ddPCR™ System for digital PCR assays – which in addition to high precision and sensitivity, has scalability to accommodate high throughput analyses.

  • Rare sequence and mutation detection
  • NGS pre- and post-run validation
  • Sensitive copy number variation (CNV) detection
  • Single cell analysis
  • Gene editing event detection
  • Gene expression and miRNA analysis
  • Linkage analysis
    • Determine if sequences are in close physical proximity
    • Results delivered via Precision’s QuartzBio platform
  • Liquid biopsy: cfDNA
  • Biodistribution
    • CAR-T
    • Gene therapy vectors
Compatible sample types include low-abundance and challenging samples
  • Tissue
  • Fresh
  • Frozen
  • FFPE
  • Cells, exosomes
  • Liquid biopsies: Blood, BM, CSF, Saliva, Tears, Urine, cfDNA, cfRNA

Our ddPCR platform also supports NGS pre- and post-run validation studies

Example ddPCR preclinical and clinical development cases

Oncology: Phase 1/2 dose escalation study in solid tumors
  • Study to determine expression level of 2 targeted genes – qPCR was requested
  • ddPCR recommended by Precision to deliver more sensitive and quantified data with less sample required
Infectious disease: Cell-free HPV DNA detection
  • Liquid biopsy, detection of HPV infection by cell-free HPV DNA
  • ddPCR ideal for detection of low abundance cell-free DNA
Gene therapy: Assess copy number in gene of interest in several tissue types
  • GLP study to determine vector copy number in 16 tissue types in NHP
  • Flexibility in sample requirements and high sensitivity in ddPCR are ideal for generating gene therapy biodistribution data
Global reach, multi-site support

Our ddPCR services support pre-clinical and clinical research, including multi-site studies, conducted anywhere in the world.

Working with Precision

Precision’s specialty lab scientists take a collaborative and consultative approach to projects and can provide recommendations on biomarker assay strategies and implementation. Services can be provided individually or as part of a comprehensive therapeutic development package including biomarker assays and clinical trials.

Related services

qPCR

Quantitative real-time PCR is a well-established and cost-effective genomic analysis technology. We utilize the QuantStudio 12k Flex with OpenArray plates for rapid, multiplexed screening of dozens of genes and samples in a single run.

NanoString Assays

With panels that enable the simultaneous analysis of up to 800 genes, Nanostring profiling is ideal for projects where highly multiplexed analysis is needed, such as oncology and autoimmune therapeutic development.

NGS

We offer NGS services including RNA-seq, whole exome sequencing, and targeted DNA/RNA sequencing services. NGS can be especially powerful when paired with our liquid biopsy technology, ApoStream.

Discuss your project requirements

Whether you know exactly the type of immune monitoring approach you need or would like expert support with project design, we can deliver.

Discuss your project requirements

Whether you know exactly the type of immune monitoring approach you need or would like expert support with project design, we can deliver.

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