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Human Peripheral Blood Mononuclear Cells (PBMCs)

Healthy or disease-state peripheral blood mononuclear cells with >95% pre-freeze viability, HLA typed and viral negative.
PBMCs are a vital component to understanding human immune function and disease response. Precision’s PBMC product inventory encompasses a large repository of healthy and disease-state cells from donors, each characterized with testing and demographic data. With a network of collection sites – we can also prospectively collect patient specimens according to your study specifications. 
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Extensively characterized PBMCs for your research

Our PBMCs are certified both clinically and molecularly to match your research endpoint.
  • Fresh or frozen PBMCs
  • Healthy and disease state samples with matched plasma
  • Highly purified cell subsets span monocytes, NK cells, T-cells, B-cells, and more
  • Cryopreserved and stored within the vapor phase liquid nitrogen (<-150ºC)
  • Consented and IRB approved for research

High-quality PBMCs accompanied by

  • High-resolution, Class I & II HLA typing
  • Demographics, medical history, and disease characterization data
  • Immunophenotyping
  • Blood pathogen testing
  • IFN-γ ELISpot responses against PHA, CEF, and CMV stimulants

How are PBMCs isolated?

PBMC isolation is the process of separating mononuclear cells from the whole blood with the use of a density gradient media and centrifugation. 

At Precision, there are three cell isolation techniques that are used to extract PBMC from the blood.

  • 1. Underlay

    1. Underlay

    Underlay Method

    A density gradient media, such as Ficoll-Paque, is used to separate the whole blood into the following components: plasma, buffy coat (mononuclear cell rich fraction), and red blood cells. When utilizing this method, the whole blood is added to the conical tube and then the density gradient media is gently added below the blood.
  • 2. Overlay

    2. Overlay

    Overlay Method

    When this method is employed, the density gradient, such as Ficoll-Paque, is placed at the bottom of the conical tube and then the whole blood sample is gently layered above the media. 
  • 3. SepMate / LeucoSep

    3. SepMate / LeucoSep

    SepMate / LeucoSep Method

    The SepMate method utilizes a SepMateTM tube that contains an insert which creates a barrier between the density gradient media and the whole blood. Similarly, the Leucosep method utilizes a special tube that contains a physical porous barrier. This allows the whole blood to be layered above the density gradient media more quickly than with traditional methods, such as underlay and overlay. The centrifuge time for these methods is shorter, resulting in more efficient PBMC isolation.

On-Site Rapid Sample Analysis for PBMCs

In conjunction with our in-house apheresis center, we have an on-site immunology lab dedicated to rapid analysis and processing of samples.

flurorescence-in-situ-hybridization

Immunophenotyping panels via flow cytometry

  • Purity assessment on isolated cell subsets
  • Custom panels available
Droplet-PCR

ELISpot/Fluorospot sample analysis

  • IFN-γ immune responses
  • Custom assays available
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Cell culture

  • PBMCs, BMMCs, DTCs
  • T-cell expansion
  • Enriched cell populations
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CBC analysis

  • 5-part differential

 

 

assays

ELISA

  • AV serotype assays
  • Custom assays available

Discover Precision's viable cell products 

Alongside our PBMCs, Precision offers various cellular products from leukopaks to purified cell subsets – collected at our in-house apheresis center.

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Viable Cell Products

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Viable Cell Products

In addition, Precision has a full-suite of global specialty lab services offering in-depth analysis for your samples

  • sample-processing

    PBMC & Sample Processing

    Sample processing with expertise in PBMC isolations from labs across 5 continents

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  • Flow Cytometry

    Standard and spectral flow cytometry, on both research-grade and CLIA-validated instruments
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  • Cell-Based Assays

    Developing and qualifying 2D and 3D human in vitro cell-based models using a broad range of human primary cell types, with analysis enabling a variety of endpoints
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