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Purified Cell Subsets

Purified immune cells, human whole blood cells, CD34+ stem cells, cord blood, DTCs, bone marrow – and other custom isolations

Cell subsets are vital to understanding human and disease biology – variability in quality, availability, and purity can derail a research study. Precision has an inventory of ready-to-ship isolated immune cells as well as a large network of collection sites and donors to prospectively collect and isolate cell subsets to meet your exact research needs. Cell subsets are purified within our global lab network.

What are purified cell subsets?

Purified cell subsets are a homogeneous cell population isolated from a larger, heterogeneous population found in samples such as whole blood, leukopaks, bone marrow, cord blood, etc.

Examples of cell subsets commonly isolated are:

  • T cells (CD3, CD4, CD8, Pan T Cells)
  • NK cells
  • Tregs
  • CD34+ stem cells
  • Plasma B cells
  • Monocytes
  • Dendritic cells
  • Neutrophils
  • Other cell isolations available upon request

How are the cell subsets purified?

While isolation techniques can be customized based on downstream applications, Precision’s immunology lab purifies cell subsets via positive or negative selection – using Miltenyi or Stem Cell Isolation Kits.

Positive selection

Cells of interest are labeled using antibody binding properties. Antibodies bound to magnetic beads are placed in the sample, allowing them to bind to surface proteins on the cells of interest. A magnetic field is then applied isolating the bound cells of interest. This results in a highly purified cell subset.

Negative selection 

Unlike positive selection, the cells of interest are left unbound in this technique, i.e. "untouched". Various antibodies bound to magnetic beads are placed in the sample, allowing them to bind to the surface proteins of all present cell populations, except the cells of interest. A magnetic field is then applied, removing the labeled, unwanted cells, resulting in an "untouched" highly purified cell subset.

Which purification technique is best for my research?

While both selection techniques result in a highly purified homogeneous cell subset, each technique could have potential benefits and drawbacks based on your downstream applications.

Positive selection targets a single type of cell and therefore results in a highly purified homogeneous population, however these cells are bound to antibodies that could interfere with cellular signaling.

Negative selection results in unbound cells of interest that have no interference in cellular signaling, but the resulting cells may be less “purified” or less homogeneous compared to positive selection, as multiple cell types have to be accurately targeted in order to remove the cells of interest.  

Microscopic transparent cells

Ready-to-ship purified cell subsets from Precision's biorepository 

  • Cell isolations from healthy donors
  • Demographics and medical history available
  • Blood pathogen testing
  • Immunophenotyping via flow cytometry
  • T cells, NK cells, Stem Cells, Monocytes, and others upon request
Lab tech

Customized cellular isolations to satisfy your downstream applications

Custom cell collections take place in our FDA Registered Blood Center and can be customized based on the following

  • Seeding density​​
  • Custom Flow panels, ELISpot / Fluorospot, ELISA, and cell culture​​
  • Cell culture optimization and characterization
  • Cryopreserved with serum free media containing DMSO or custom cryopreservation media
  • Disease-state cellular isolations available as custom collections only

Case Study - Custom T Cell Isolation

Isolation of CD4+ and CD8+ cells from a fresh normal healthy leukopak using magnetic beading technology from Miltenyi

  • Cells bulk frozen in a CryoBag and shipped to cleint
  • Purity Check completed for the following:
    • Viability
    • CD3
    • CD4
    • CD8
Flow_CD3CD4CD8

Discover Precision's viable cell products 

Alongside our purified cell subsets, Precision offers various cellular products from PBMCs to leukopaks and more – collected at our in-house apheresis center.

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Viable Cell Products

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Viable Cell Products

Access to deep characterization of your purified cell subsets with Precision's global specialty labs

  • Flow Cytometry

    Standard and spectral flow cytometry, on both research-grade and CLIA-validated instruments
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  • sample-processing

    PBMC & Sample Processing

    Sample processing with expertise in PBMC isolations from labs across 5 continents

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  • Cell-Based Assays

    Developing and qualifying 2D and 3D human in vitro cell-based models using a broad range of human primary cell types, with analysis enabling a variety of endpoints
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